BIOTEC Dresden/Notepad/30 August 2010

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Revision as of 13:50, 24 October 2010 by Lucas.schirmer (Talk | contribs)

Fusion Protein

The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour. The whole ligation reaction was used for electroporation.


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