BIOTEC Dresden/Notepad/18 August 2010

From 2010.igem.org

Revision as of 21:08, 18 August 2010 by Vyctoryo (Talk | contribs)

The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours followed by heat inactivation.

Digestion was followed by a second purification with the pcr purification kit, concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the band seemed rather weak and was accompanied by a smear)





July
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
August
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
September
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
October
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Share to Twitter Share to Facebook Share to Orkut Stumble It Email This More...