BIOTEC Dresden/Notepad/18 August 2010

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'''Parts Assembly'''
The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours after which heat inactivation was performed.
The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours after which heat inactivation was performed.
This was followed by a second purification with the pcr purification kit, by concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the bands seemed rather weak and were accompanied by a smear)
This was followed by a second purification with the pcr purification kit, by concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the bands seemed rather weak and were accompanied by a smear)
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[[Category:BIOTEC Dresden/Notepad/August|August]]
{{Biotec_Dresden/month}}
{{Biotec_Dresden/month}}
{{Biotec_Dresden/Bottom}}
{{Biotec_Dresden/Bottom}}

Latest revision as of 21:59, 27 October 2010

Parts Assembly

The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours after which heat inactivation was performed.

This was followed by a second purification with the pcr purification kit, by concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the bands seemed rather weak and were accompanied by a smear)


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