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BIOTEC Dresden/Notepad/21 September 2010
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Parts Assembly
PCR amplification of the following parts and the plasmid backbone containing chloramphenicol was done.
4a, 9b, 14f, 20f, 21f
The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated.
Fusion Protein
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.
July |
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