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BIOTEC Dresden/Notepad/2 September 2010
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Five colonies for each of the following parts were picked and colony PCR was performed.
4a, 14f, 20f, 21f
Positive clones were identified and the corresponding cultures were left for overnight shaking.
Gradient PCR of primers for the parts and the chloramphenicol backbone was done and the right annealing temperature was determined.
4a, 14f, 20f, 21f
Fusion Protein
To varify the correct insertion plasmids from positive colony PCR were sequenced.
July |
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August |
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October |
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