Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/15

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2010/08/15

1：DNA extraction with ISOPLANT(NIPPON GENE)

＜Member＞
Mariko, easily, nito, Hitomi, Taka


＜Sample＞
・E-coli (having BBa_I732901 plasmid)


＜Protocol＞
SeeProtocol 5


2：DNA concentration measurement

＜Member＞
Mariko, easily, nito, Hitomi, Taka


＜Materials, Sample＞
・TE

・E-coli (having BBa_I732901 plasmid)


＜Protocol＞

  1. Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
  2. Fall in drops 1 on the measuring machine.


＜Result＞
Concentration: 16.8
A320: 0.046
A260/A280: 3.414
A260/A230: 0.062

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