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Team:Newcastle/20 August 2010
From 2010.igem.org
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Contents |
Subtilin Immunity
Gel Electrophoresis and Gel Extraction
Aims
Following yesterday's Phusion PCR reactions, we plan to first perform gel electrophoresis of the amplified DNA sequences to check if they are of the correct sizes. If they appear to be successful we will then perform gel extraction, and finally nanodrop to record the level of DNA.
Materials and protocol
Please refer to the:
- gel electrophoresis,
- gel extraction and
- NanoDrop spectrophotometer protocols.
Results
Discussion
Conclusion
PCR of pSB1C3 for rocF and Subtilin Immunity
Aims
Gel Extraction
Aims
To purify the PCR products of digested yneA, pGFPrrnB and pSB1C3 from yesterday.
Materials and Protocol
Please refer to gel extraction and nanodrop.
Results
Discussion
Conclusion
Miniprep of yneA, pGFPrrnB and pSB1C3
Aim
To produce stocks of yneA, pGFPrrnB and pSB1C3.
Materials and Protocol
Please refer to miniprep and nanodrop.
Results
Discussion
Conclusion
Transformation of Ligated Products
Aims
To produce more colonies of the ligated products of yneA with pGFPrrnB and pSB1C3.
Materials and Protocol
Please refer to transformation of E. coli.
Ligation of yneA with Vectors
Aims
To ligate yneA with pGFPrrnB and yneA with pSB1C3 (A repeat of yesterday.
Materials and Protocol
Please refer to ligation.
Results, Discussion and Conclusion
Please refer to 23.08.10.
   
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