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Contents 1 Subtilin Immunity 1.1 Gel Electrophoresis and Gel Extraction 1.1.1 Aims 2 PCR of pSB1C3 for rocF and Subtilin Immunity 2.1 Aims 2.2 Materials and protocol 3 Gel Extraction 3.1 Aims 3.2 Materials and Protocol 3.3 Results 3.4 Discussion 3.5 Conclusion 4 Miniprep of yneA, pGFPrrnB and pSB1C3 4.1 Aim 4.2 Materials and Protocol 4.3 Results 4.4 Discussion 4.5 Conclusion 5 Transformation of Ligated Products 5.1 Aims 5.2 Materials and Protocol 6 Ligation of yneA with Vectors 6.1 Aims 6.2 Materials and Protocol 6.3 Results, Discussion and Conclusion

Subtilin Immunity

Gel Electrophoresis and Gel Extraction

Aims

To run the amplified PCR products from yesterday's PCR reactions were ran on a gel

PCR of pSB1C3 for rocF and Subtilin Immunity

Aims

Following yesterday's Phusion PCR, we plan to first perform gel electrophoresis, then gel extraction, and finally nanodrop to record the level of DNA.

Materials and protocol

Please refer to the gel electrophoresis and gel extraction protocols.

Gel Extraction

Aims

To purify the PCR products of digested yneA, pGFPrrnB and pSB1C3 from yesterday.

Materials and Protocol

Please refer to gel extraction and nanodrop.

Results

Discussion

Conclusion

Miniprep of yneA, pGFPrrnB and pSB1C3

Aim

To produce stocks of yneA, pGFPrrnB and pSB1C3.

Materials and Protocol

Please refer to miniprep and nanodrop.

Results

Discussion

Conclusion

Transformation of Ligated Products

Aims

To produce more colonies of the ligated products of yneA with pGFPrrnB and pSB1C3.

Materials and Protocol

Please refer to transformation of E. coli.

Ligation of yneA with Vectors

Aims

To ligate yneA with pGFPrrnB and yneA with pSB1C3 (A repeat of yesterday.

Materials and Protocol

Please refer to ligation.

Results, Discussion and Conclusion

Please refer to 23.08.10.