Team:Caltech/Week 10

Monday 8/16

• Group meeting!
• Ran CPCR gels on L31, L34, L43, L58 & L71
  • All negative.

Tuesday 8/17

• Took our team photo!
• Ran more CPCR of L58 & L81.
  • Gel was negative.
• Cultured HSP-LacI-GFP cells in LB + 1M glucose to try to up-regulate LacI repression of GFP production. Incubated cells at 37°C overnight.

Wednesday 8/18

• LacI appears fully repressed in L62 cells after overnight incubation at 37°C.
  • Will now spin down cells and resuspend in LB + IPTG to see if overnight incubation now activates GFP production, as predicted.
• Picked more CPCR colonies of L81 & L58 (x6) to try to find plastic production cells.
• Transformed L60 & L61. Plated on plates containing 50μL 50mM glucose (dried).
  • We hypothesize that the reason these ligations produced no colonies is due to leaky LacI repression, allowing a low level of lysis expression, eventually killing all the cells. Will see if there are cells on these plates tomorrow.
• Submitted our poster deign for printing for Sasha's SURF presentation tomorrow.

Thursday 8/19

Friday 8/20