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BIOTEC Dresden/Notepad/10 August 2010
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Check Gradient PCR of all primers on plasmids on agarose gel:
23e, 25a, 27e
Except two parts 20f and 21f, all the other plates had sufficient growth of colonies. Hence, colony PCR of the same was carried out for which eight single colonies were picked for each part.
4a, 13e, 9b, 15e, 14f
Agarose gel electrophoresis was performed to check the positive clones from the PCR products. Unfortunately, the gel ran too long because of which we did not have any results for this gel. Hence, the same parts were again prepared for colony PCR to be done on 11.08.2010 and this time, five colonies for each part was picked.
The following parts were transformed by electroporation:
10, 11, 42, 43
The samples from the overnight ligation were also transformed by electroporation and plated.
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