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BIOTEC Dresden/Notepad/9 August 2010
From 2010.igem.org
Check gradient PCR with Taq polymerase from 08.08.2010 on agarose gel on plasmids:
23e, 25a, 27e
Gradient PCR using Fusion polymerase on plasmids:
23e, 25a, 27e
Assembly of the following parts were commenced with restriction digest and ligation:
6e, 4a, 13e, 9b, 15e, 14f, 20f, 21f
The plasmid backbone used was pSB1C3. Two ligations were performed - one based on the standard protocol and the other samples were left for overnight ligation. The ligated parts were transformed by electroporation method and plated.
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