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From 2010.igem.org

We present our modeling details of this AHL-induced LuxR-regulated lux pR-gfp system. We assume:

• LuxR does not form dimer;
• AHL binds to LuxR and forms AHL-LuxR complex;
• AHL-LuxR could form dimer, (AHL-LuxR)2;
• Only (AHL-LuxR)2 could activate transcription from promoter lux pR;

There are 12 species in total:

• 0:E_coli: E.coli cell in flask;
• 1:AHL: AHL added in the flask;
• 2:DNA: plasmid transformed into E.coli at the beginning;
• 3:AHL: AHL in E.coli because of diffusion;
• 4:RNA: mRNA of GFP;
• 5:RNA: mRNA of LuxR;
• 6:E0040_GFP: GFP protein;
• 7:C0062_LuxR: LuxR protein;
• 8:E0040_GFP_mature: mature GFP protein;
• 9:AHL;C0062_LuxR: complex of AHL and LuxR;
• 10:(AHL)2;(C0062_LuxR)2: dimer of complex AHL-LuxR;
• 11:(AHL)2;(C0062_LuxR)2;DNA: binding complex of AHL-LuxR dimer and lux pR on plasmid DNA;

There are 39 reactions in total. A screenshot is provided below: