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Team:Newcastle/18 August 2010
From 2010.igem.org
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Contents |
Miniprep of yneA BioBrick
Aims
The aim of the experiment is to extract yneA in the biobrick comapatible vector from 17th August, 2010 overnight culture.
Materials and Protocol
Please refer to protocols mentioned below for materials required:
Results
| yneA 1 | yneA 2 | yneA 3 | yneA 4 | |
|---|---|---|---|---|
| Concentration of DNA ng/µl | 247.3 | 233.6 | 456.6 | 140.0 |
Table 1: Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.
Figure 1: Screenshot of yneA tube 1
Figure 2: Screenshot of yneA tube 2
Figure 3: Screenshot of yneA tube 3
Figure 4: Screenshot of yneA tube 4
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Discussion
The concentration of the different yneA tubes range from 140.0 µl/ml to 456.6 µl/ml. The standard value for miniprep extraction is 150 µg/ml.
Conclusion
High concentration of yneA were obtained.
   
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