Revision as of 20:21, 25 October 2010 by Theotime (Talk | contribs)

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Glycerols

• Ps-MTet x2
• Ps-STet x2
• PsS-WTet-LuxI 1 and 2
• 6h A+ PmS
• 5h A+ psS
• 6h ctrl A PmS
• 5h ctrl A PsS
• 5h ctrl A PmS
• 4h ctrl A PsS

Minipreps

• 6h A+ PmS
• 5h A+ psS
• 6h ctrl A PmS
• 5h ctrl A PsS
• 5h ctrl A PmS
• 4h ctrl A PsS
• PsS-WTet-LuxI 1 and 2
• Ps-MTet 1 and 2
• Ps-STet 1 and 2

Gel electrophoresis with PCR from 09/10
many bands on the gel but the 4Kb band is seen (PmS integrated into the chromosome ?)

PCR to verify that the terminator is out in our A+ constructs (primers : VF2 + VR)

• 6h A+ PmS
• 5h A+ psS
• 6h ctrl A PmS
• 5h ctrl A PsS
• 5h ctrl A PmS
• 4h ctrl A PsS

Gel electrophoresis

• 6h A+ PmS
• 5h A+ psS
• 6h ctrl A PmS
• 5h ctrl A PsS
• 5h ctrl A PmS
• 4h ctrl A PsS

strange results

Ligations (V + I)

• 1) PmS-MTet-LuxI + I13507/pLux/GFP
• 2) pLux/LuxI + RBS-GFP-tt (E0240)
• 3) pLux + LuxI
• 4) Ctrl PmS-MTet-LuxI
• 5) Ctrl pLux/LuxI
• 6) Ctrl pLux

Transformation of 1-6

Overnight cultures

• PmS Chr + pBad/Int x2