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Théotime
Gel extraction from yesterday

• Pss/wTet/LuxI (9)
• Pss/wTet/LuxI (10)
• Pms/mTet/LuxI (7)
• Pss/sLac/LuxI (17)
• Pms/sLac/LuxI (18)

Aleksandra
Ligations for cheracterization : same as 03/09, started with the same digestions, burt once again

• sTet+Ps
• mTet+Ps
• wTet+Ps

Raphaël
Ligations

• pSB1C3 + Pss/wTet/LuxI (9)
• pSB1C3 + Pss/sLac/LuxI (17)
• pSB1C3 + Pms/mTet/LuxI (7)
• pSB1C3 + Pss/wTet/LuxI (10)
• pSB1C3 + Pss/sLac/LuxI (18)
• pSB1C3 ctrl

Transformations in TURBO cells

• pSB1C3 + Pss/wTet/LuxI (9)
• pSB1C3 + Pss/sLac/LuxI (17)
• pSB1C3 + Pms/mTet/LuxI (7)
• pSB1C3 + Pss/wTet/LuxI (10)
• pSB1C3 + Pss/sLac/LuxI (18)
• pSB1C3 ctrl

Overnight cultures

• sLac/LuxI III
• LuxI
• RBS/GFP/tt (E0240)
• pLux
• Pss/wTet/LuxI (10), but it didn't grow
• Pms/mTet/LuxI (7)
• Pss/sLac/LuxI III (17), but it didn't grow
• I13507/pLux/GFP
• attP/Pms/attC/RFP, col 1
• attP/Psm/attC/RFP, col 1
• attP/Pss/attC/RFP, col 1
• Pms/mTet/LuxI chr
• Pss/wTet/LuxI chr, but it didn't grow
• pUC chr, but it didn't grow
• PswTet, colonies 1-4 for characterisation
• PsmTet, colonies 1-4 for characterisation
• PssTet, colonies 1-4 for characterisation

Touchdown PCR overnight

• PswTet, colonies 1-4 for characterisation
• PsmTet, colonies 1-4 for characterisation
• PssTet, colonies 1-4 for characterisation

But there was a problem with the PCR machine, it didn't work.