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Raphaël
Minipreps

• Ps/sRBS/LuxR/attC/t/attC/mRFP
• Ps/mRBS/LuxR/attC/t/attC/mRFP
• Ps/wRBS/LuxR/attC/t/attC/mRFP

Restriction digest with SpeI and PstI : 20µl miniprep + 2.5µl B2 + 2x1µl enzymes + 0.25µl BSA

• Ps/sRBS/LuxR/attC/t/attC/mRFP
• Ps/mRBS/LuxR/attC/t/attC/mRFP
• Ps/wRBS/LuxR/attC/t/attC/mRFP

Gel electrophoresis

• Ps/wRBS/LuxR/attC/t/attC/mRFP
• -
• Ps/mRBS/LuxR/attC/t/attC/mRFP
• -
• Ps/sRBS/LuxR/attC/t/attC/mRFP
• Ladder 1kb

<a href="/index.php?title=Special:Upload&wpDestFile=2308_1.jpg" class="new" title="Image:2308 1.jpg">Image:2308 1.jpg</a>

Gel extraction

• Ps/sRBS/LuxR/attC/t/attC/mRFP
• Ps/mRBS/LuxR/attC/t/attC/mRFP

Aleksandra
PCR, 1µl miniprep (13/08) + 2x1µl primers 0.025ng/µl + 10µl water + 13µl 2x quick load mix, Tm=60, Telong=68

• wRBS/TetA(C)
• mRBS/TetA(C)
• sRBS/TetA(C)

Gel electrophoresis

• wRBS/TetA(C)
• mRBS/TetA(C)
• sRBS/TetA(C)
• Ladder 1Kb

<a href="/index.php?title=Special:Upload&wpDestFile=2308_2.jpg" class="new" title="Image:2308 2.jpg">Image:2308 2.jpg</a>
-> bands of agarose in the fridge