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Léa
Restriction digest with XbaI and PstI : 5µl miniprep + 15µL water + 2x1µl enzyme + 2.5µl B3 + 0.25µl BSA

• 3x Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI
• 4x Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI
• 1x Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI
• 1x Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI
• 2x Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI
• 2x Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI
• 3x pLux/LuxI

Gel electrophoresis

• Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony II
• Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony III
• Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony IV
• Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony I
• Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony II
• Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony III
• Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony IV
• Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI, colony I
• Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
• Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
• Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, colony II
• Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
• Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, colony IV
• pLux/LuxI, colony I
• pLux/LuxI, colony II
• pLux/LuxI, colony III
• Ladder 1kb

<a href="/index.php?title=Special:Upload&wpDestFile=Gel1908_1.jpg" class="new" title="Image:Gel1908 1.jpg">Image:Gel1908 1.jpg</a>

Aleksandra

Overnight cultures

• Ps/wRBS/LuxR + attC/term/attC/mRFP
• Ps/mRBS/LuxR + attC/term/attC/mRFP
• Ps/sRBS/LuxR + attC/term/attC/mRFP
• Pm/sRBS/LuxR + attC/term/attC/mRFP
• pLux
• wRBS/LacZ
• sRBS/LacZ
• wRBS/TetA(C)
• mRBS/TetA(C)
• sRBS/TetA(C)
• 2x RBS/LuxI