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Aleksandra
Transformations of TURBO cells with 5µl ligation product, plating with 150µl

• 1)Pm/sRBS/LuxR + wRBS/TetA(C)/LuxI
• 2)Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI
• 3)Ps/mRBS/LuxR + wRBS/TetA(C)/LuxI
• 4)Ps/sRBS/LuxR + wRBS/TetA(C)/LuxI
• 5)Pm/sRBS/LuxR + mRBS/TetA(C)/LuxI
• 6)Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI
• 7)Ps/mRBS/LuxR + mRBS/TetA(C)/LuxI
• 8)Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI
• 9)Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI
• 10)Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI
• 11)Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI
• 12)Ps/sRBS/LuxR + sRBS/TetA(C)/LuxI

Controls:

• 13)Pm/sRBS/LuxR
• 14)Ps/wRBS/LuxR
• 15)Ps/mRBS/LuxR
• 16)Ps/sRBS/LuxR
• 17)pUC19 (positive control)

Léa
Glycerols and minipreps

• wRBS/LacZα/LuxI, 2 cultures
• sRBS/LacZα/LuxI, 2 cultures
• LuxI

Nanodrop of the minipreps

Sample name	260/280	260/230	ng/µl
wRBS/LacZα/LuxI cult1	1.90	2.40	422.1
wRBS/LacZα/LuxI cult2	1.95	2.34	362.3
sRBS/LacZα/LuxI cult1	1.92	2.37	387.4
sRBS/LacZα/LuxI cult2	1.94	2.35	415.2
LuxI	1.94	2.37	207.0