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Aleksandra
Transformation of TURBO cells with yesterday's ligations using 5µl ligation product to transform 50µl cells, then plating on Amp+LB with 150µl LB+transformation.

• mRBS/TetA(C)+RBS/LuxI
• wRBS/TetA(C)+RBS/LuxI
• stRBS/TetA(C)+RBS/LuxI
• stRBS/LacZα+RBS/LuxI
• RBS/mRFP/t(K081014)+pLux/GFP
• RBS/mRFP/tt(I13507)+pLux/GFP
• Pm/wRBS/LuxR +attC/term/attC/mRFP
• Ps/wRBS/LuxR +attC/term/attC/mRFP
• Pm/mRBS/luxR +attC/term/attC/mRFP
• Ps/mRBS/LuxR +attC/term/attC/mRFP
• Pm/sRBS/LuxR +attC/term/attC/mRFP
• Ps/sRBS/LuxR +attC/term/attC/mRFP

Controls:

• RBS/LuxI
• pLux/GFP
• Pm/wRBS/LuxR
• Ps/wRBS/LuxR
• Pm/mRBS/luxR
• Ps/mRBS/LuxR
• Pm/sRBS/LuxR
• Ps/sRBS/LuxR
• Positive control (pUC19)

All the transformation plates have colonies

verification of glycerols : plating

• wRBS/tetA(C)
• mRBS/TetA(C)
• stRBS/LacZα
• Pm
• Ps
• Ps/wRBS/LuxR

All plates have a lot of colonies, the glycerols are OK

Théotime

Make Plate 2x 500 mL of LBA + AMP : 2x 19 plates.

Aleksandra

Starting overnight cultures, 12ml cultures LB+Amp

• mRBS/TetA(C)+RBS/LuxI, 4 colonies
• wRBS/TetA(C)+RBS/LuxI, 4 colonies
• stRBS/TetA(C)+RBS/LuxI, 4 colonies
• RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies
• RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies
• Pm/wRBS/LuxR +attC/term/attC/mRFP, 1 possible colony, didn't grow
• Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies
• Pm/mRBS/luxR +attC/term/attC/mRFP, 1 possible colony, didn't grow
• Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies
• Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony
• Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies