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Aleksandra
Minipreps, elution in 50µl warm water

• Pst/weakRBS/LuxR
• Pst/medRBS/LuxR
• Pst/stRBS/LuxR
• Pmed/weakRBS/LuxR
• Pmed/medRBS/LuxR
• Pmed/stRBS/LuxR
• Pmed
• Pst
• K081014, colony2
• I13507, colony2
• I13507, colony3
• pBAD/Int1 (Amp, glycerol45)
• pBAD/Int1 (Kan, glycerol 18)
• LuxI
• pLux/RBS/GFP

Nanodrop

Sample name	260/280	260/230	ng/µl
K081014, col2	1.94	2.13	222.8
I13507, col2	1.92	2.01	220.4
I13507, col3	1.96	1.99	187.7
pLux/RBS/GFP/term	1.91	2.09	253.4
RBS/LuxI	1.93	1.85	140.3
Pmed/weakRBS/LuxR	1.92	1.84	138.8
Pst/weakRBS/LuxR	2.00	2.10	117.3
Pmed/medRBS/LuxR	1.93	2.09	210.6
Pstr/medRBS/LuxR	2.00	2.20	153.5
Pmed/strRBS/LuxR	1.86	1.76	122.8
Pstr/strRBS/LuxR	2.03	2.21	145.6
Pmed	1.86	1.99	179.7
Pstr	1.98	2.06	181.4
Pbad/IntI (glycerol S45)	1.95	1.90	140.4
Pbad/IntI (glycerol S18)	2.02	2.35	152.4