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Théotime
Glycerol 15%, -20°C and -80°C stock per pop.

• Pm/weakRBS/LuxR/attC/term/attC/RFP (1-2-3)
• Ps/weakRBS/LuxR/attC/term/attC/RFP (1-2)
• Ps/medRBS/LuxR/attC/term/attC/RFP (1-2) (error on gly.2 -> Media is clear, there's no bacteria, I didn't see)
• Pm/stdRBS/LuxR/attC/term/attC/RFP (1-2)
• Ps/RFP (1-2-3-4)

Xavier

MiniPrep in 40 µL water.

• Pm/weakRBS/LuxR/attC/term/attC/RFP (1-2-3)
• Ps/weakRBS/LuxR/attC/term/attC/RFP (1-2)
• Ps/medRBS/LuxR/attC/term/attC/RFP (1)
• Pm/stdRBS/LuxR/attC/term/attC/RFP (1-2)

Théotime

Miniprep in 40µL water.

• Ps/RFP (1-2-3-4)

Nanodrop

Set values here.

Restriction Digest with Xba1 (1µL) and Pst1(1µL), in 2.5µL Buffer 3, 0.25µL BSA and 5µL water for 15µL miniprep.

(Note : Total=25µL, but I got ~30µL, I've made an error in my manip ...)

• Pm/weakRBS/LuxR/attC/term/attC/RFP (1-2-3)
• Ps/weakRBS/LuxR/attC/term/attC/RFP (1-2)
• Ps/medRBS/LuxR/attC/term/attC/RFP (1)
• Pm/stdRBS/LuxR/attC/term/attC/RFP (1-2)

Gel Electro with 50µL tBE and 0.75g Agarose (1.5%) and 1.2µL BET, in tBE 0.5X.

• Pm/weakRBS/LuxR/attC/term/attC/RFP (1-2-3)
• Ps/weakRBS/LuxR/attC/term/attC/RFP (1-2)
• Ps/medRBS/LuxR/attC/term/attC/RFP (1)
• Pm/stdRBS/LuxR/attC/term/attC/RFP (1-2)
• empty
• empty
• 1kb ladder

The dye forms a wave... I've stop the gel when 1kb ladder touch the last line, but the "wave" don't.

<a href="/wiki/Image:Paris_gel_08m07d2010y.jpg" class="image" title="Paris gel 08m07d2010y.jpg"><img alt="" src="/images/thumb/7/75/Paris_gel_08m07d2010y.jpg/500px-Paris_gel_08m07d2010y.jpg" width="500" height="374" border="0" /></a>

Culture Overnight in 12mL AMP.

• K081014
• I13507
• Ps/medRBS/attC/term/attC/RFP (3)
• Pm/weakRBS/attC/term/attC/RFP (4)
• Ps/stdRBS/attC/term/attC/RFP (2)