Team:Paris Liliane Bettencourt/Notebook/2010/08/05/
From 2010.igem.org
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Léa
Minipreps of 10 ml cultures, elution in 30µl warm water
- attC/term/attC/mRFP, 11 minipreps from glycerol
- RBS/LuxI/term, started from colony 1 from TURBOcell plate 1
- RBS/LuxI/term, started from colony 1 from TURBOcell plate 2
- RBS/LuxI/term, started from colony 2 from TURBOcell plate 2
- RBS/LuxI/term, started from colony 1 from TOP10 plate
- RBS/LuxI/term, started from colony 1 from TOP10 plate
- term from glycerol
DNA quantification in the minipreps with the Nanodrop
Sample name | 260/280 | 260/230 | ng/µl |
attC/term/attC/mRFP | 1.99 | 1.98 | 206.8 |
RBS/LuxI/term (TURBO1 col1) | 1.94 | 2.22 | 318.6 |
RBS/LuxI/term (TURBO2 col1) | 1.95 | 2.27 | 325.4 |
RBS/LuxI/term (TURBO2 col2) | 1.95 | 2.31 | 364.1 |
RBS/LuxI/term (TOP10 col1) | 1.96 | 1.99 | 246.5 |
RBS/LuxI/term (TOP10 col2) | 1.93 | 2.17 | 292.4 |
term | 1.98 | 2.20 | 299.9 |