Team:Paris Liliane Bettencourt/Notebook/2010/08/05/

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Léa
Minipreps of 10 ml cultures, elution in 30µl warm water

  • attC/term/attC/mRFP, 11 minipreps from glycerol
  • RBS/LuxI/term, started from colony 1 from TURBOcell plate 1
  • RBS/LuxI/term, started from colony 1 from TURBOcell plate 2
  • RBS/LuxI/term, started from colony 2 from TURBOcell plate 2
  • RBS/LuxI/term, started from colony 1 from TOP10 plate
  • RBS/LuxI/term, started from colony 1 from TOP10 plate
  • term from glycerol


DNA quantification in the minipreps with the Nanodrop

Sample name 260/280 260/230ng/µl
attC/term/attC/mRFP 1.991.98 206.8
RBS/LuxI/term (TURBO1 col1)1.94 2.22 318.6
RBS/LuxI/term (TURBO2 col1) 1.95 2.27325.4
RBS/LuxI/term (TURBO2 col2) 1.95 2.31364.1
RBS/LuxI/term (TOP10 col1) 1.96 1.99246.5
RBS/LuxI/term (TOP10 col2) 1.932.17 292.4
term 1.982.20 299.9