Team:Paris Liliane Bettencourt/Notebook/2010/08/03/
From 2010.igem.org
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Théotime and Raphaël
Transformation of TURBO cells, incubation at 37°C around 10h
- RBS/LuxI/term (ligation of 26/07), with 50µl
- RBS/LuxI/term (ligation of 26/07), with 100µl
- positive control
Aleksandra
Minipreps of 10ml, elution in 35µl warm water
- attC (from glycerol stock)
- term/attC/mRFP (from glycerol stock)
- attC/term/attC/mRFP #10 (from yesterday's glycerol stock)
- attC/term/attC/mRFP #11 (from yesterday's glycerol stock)
DNA quantification in the minipreps using the nanodrop
Sample name | 260/280 | 260/230 | ng/µl |
attC | 2.13 | 1.69 | 135.2 |
term | 2.13 | 2.07 | 169.9 |
attC/term/attC/mRFP #10 | 2.02 | 1.91 | 118.0 |