Team:Paris Liliane Bettencourt/Notebook/2010/07/30/
From 2010.igem.org
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Aleksandra
Gel purification with the Quiagen kit
Purification of the bands extracted yesterday :
- term/attC/mRFP
- weak.RBS /TetA(C)
- med.RBS /TetA(C)
- stand.RBS /TetA(C)
- weak.RBS /LacZ
- stand.RBS /LacZ
Léa
PCR purification with the Quiagen kit
- attC
- LuxI/term
Léa, Aleksandra and Raphaël
Nanodrop
Quantification of DNA amounts in the minipreps from yesterday
Sample name | 260/280 | 260/230 | ng/µl |
stand.RBS /TetA(C) 1 | 1.93 | 2.31 | 271.3 |
pLux/RBS/GFP/term A | 1.90 | 2.18 | 244.8 |
Pmed/weakRBS/LuxR 1 | 1.91 | 1.52 | 87.8 |
Pstr/weakRBS/LuxR 1 | 1.94 | 2.28 | 88.4 |
Pmed /medRBS/LuxR 1 | 1.89 | 2.19 | 156.2 |
Pstr/medRBS/LuxR 1 | 1.91 | 2.04 | 83.9 |
Pmed/strRBS/LuxR 1 | 1.94 | 2.37 | 236.5 |
Pstr/strRBS/LuxR 1 | 1.96 | 2.43 | 101.3 |
stand.RBS/LacZ 1 | 1.88 | 1.68 | 224.7 |
weak RBS/LacZ 1 | 1.92 | 2.06 | 162.4 |
LuxI/term 1 | 1.91 | 2.18 | 325.2 |
attC | 1.97 | 1.96 | 152.0 |
term/attC/mRFP | 2.03 | 2.36 | 213.2 |