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Miniprep

• S200 (Constitutif promoter + standard RBS + tetR) from iGEM08 Vf=60 uL 72.9 ng/ul
• S173 (standard RBS + tetR)from iGEM08 Vf=60 uL 208.3 ng/uL

Restriction digest Vf=20 uL

Sample name	DNA (1ug)	enzyme	Buffer	H2O	BSA
S173	4.8	1 S/P	2(B2)	11	0.2
J23110 (Vf=40uL)	2	2 S/P	4(B2)	29.6	0.4
B0015	7.4	2 X/P	4 (B2)	24.2	0.4
P1003	2	1 S/P	2 (B2)	13.8	0.2
C0012	6.5	1 S/P	2 (B2)	9.3	0.2