Team:Paris Liliane Bettencourt/Notebook/2010/07/17/
From 2010.igem.org
Aleksandra
Minipreps of the overnight cultures (Qiagen kit)
2 series of minipreps of 5 ml culture each :
- attC - Kan
- term-attC-mRFP - Kan
- term (B0015) - Amp
- RBS/GFP/term (E0240) - Amp
- RBS/LuxR (J37033) - Amp
- pLux (R0062) - Amp
- RBS/LuxI (K081008) - Amp
- Pstrong (J23119) - Amp
- Pmedium (J23110) - Amp
DNA from both minipreps is collected into the same tube in the end -> 2x30 µL = 60 µL DNA of each plasmid in the end