Raphaël and Théotime

DNA Gel electrophoresis (agarose 1,0% w/v)

2 µL of EtBr was added in the gel

Protocol (3 wells)

• 10 µL of minipreped DNA (Qiagen - attC) + 2 µL of loading buffer 6x
• 10 µL of minipreped DNA (Promega - attC) + 2 µL of loading buffer 6x
• 10 µL of ladder 1Kb

Migration : 50V, 1h

<a href="/wiki/Image:Electrophoresis_070710.jpg" class="image" title="Image:electrophoresis_070710.jpg"><img alt="Image:electrophoresis_070710.jpg" src="/images/7/7e/Electrophoresis_070710.jpg" width="436" height="498" border="0" /></a>

The result is not really conclusive...

Overnight culture

10mL LB

• pSUlib EX-B0015-attC-S-mRFP1-P (+Kan) 37°C
• pBad18-IntI1 (+Amp +1% glucose) 37°C
• pSWK attP-1E (+Cm +dT) 37°C
• Pi1(+dT) 37°C
• pTSA CX1 Int lambda (+Amp) 25°C

Plates in the fridge

Antoine

Preparation of plates (Amp + Kan)