Team:Paris Liliane Bettencourt/Notebook/2010/07/07/

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Raphaël and Théotime

DNA Gel electrophoresis (agarose 1,0% w/v)


2 µL of EtBr was added in the gel

Protocol (3 wells)

  • 10 µL of minipreped DNA (Qiagen - attC) + 2 µL of loading buffer 6x
  • 10 µL of minipreped DNA (Promega - attC) + 2 µL of loading buffer 6x
  • 10 µL of ladder 1Kb

Migration : 50V, 1h

<a href="/wiki/Image:Electrophoresis_070710.jpg" class="image" title="Image:electrophoresis_070710.jpg"><img alt="Image:electrophoresis_070710.jpg" src="/images/7/7e/Electrophoresis_070710.jpg" width="436" height="498" border="0" /></a>


The result is not really conclusive...

Overnight culture


10mL LB

  • pSUlib EX-B0015-attC-S-mRFP1-P (+Kan) 37°C
  • pBad18-IntI1 (+Amp +1% glucose) 37°C
  • pSWK attP-1E (+Cm +dT) 37°C
  • Pi1(+dT) 37°C
  • pTSA CX1 Int lambda (+Amp) 25°C

Plates in the fridge


Antoine

Preparation of plates (Amp + Kan)