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BIOTEC Dresden/Notepad/21 September 2010
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Parts Assembly
PCR amplification of the following parts and the plasmid backbone containing chloramphenicol was done.
4a, 9b, 14f, 20f, 21f
The PCR products were run on an agarose gel. Due to difference in buffer usage, this step was once again repeated.
Fusion Protein
Ligation of the parts was carried out followed by their transformation. Gradient PCR for the fusion parts was done.
July |
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August |
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September |
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October |
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