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Contents 1 PCR/pBS1C3 1.1 member 1.2 materials,Procedure 2 Agarose Gels Electro-phoresis 2.1 member 2.2 Materials,Procedure 3 Experiment:Make Agarose Gels Electro-phoresis 3.1 Member 3.2 Materials and Procedure 4 Medium making 4.1 member 4.2 materials and procedure 5 Ligation 5.1 member 5.2 materials and procedure

PCR/pBS1C3

member

naoto and bambi75

materials,Procedure

Same on August 23th.

• 95℃3min
• 96℃1min
• 51℃1min
• 72℃3min
• 10℃∞

（96℃～72℃/30cycle）

Agarose Gels Electro-phoresis

member

Same above

Materials,Procedure

Same protocol on August 16th. PCR Product of insert is pBS1C3

Experiment:Make Agarose Gels Electro-phoresis

Member

Same above

Materials and Procedure

Same protocol on August 16th.

Medium making

member

Same above

materials and procedure

Same protocol on August 10th.

Ligation

member

Same above

materials and procedure

follow the protocol(Ligation).