User contributions

From 2010.igem.org

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• 00:38, 24 October 2010 (diff | hist) N Minnesota-experimental/21 July 2010 ‎ (New page: '''Annie''' Inoculated four microliters Pichia in fifty microliters YPD media. Plated on YPD media for future inoculation. '''Tony and Matt''' Colony PCR screen to find pacBB EutG DsRed c...) (top)
• 00:34, 24 October 2010 (diff | hist) Minnesota-experimental/20 July 2010 ‎ (top)
• 00:32, 24 October 2010 (diff | hist) N Minnesota-experimental/19 July 2010 ‎ (New page: '''Tony''' Ligation of pacBB EutG DsRed. Cultivated two colonies of pacBB EutC DsRed in LB and left overnight at thirty seven degrees Celsius. '''Ian''' Transformation of psB3K3 Eut LKMNS...) (top)
• 00:29, 24 October 2010 (diff | hist) N Minnesota-experimental/16 July 2010 ‎ (New page: '''Ian''' Ligation of Eut LKMNS with psb3K5 (twenty microliters).) (top)
• 00:28, 24 October 2010 (diff | hist) N Minnesota-experimental/20 July 2010 ‎ (New page: '''Annie''' Made YDP media and inoculated PIchia Pastoris. '''Tony''' PacBB GGFP digest with BglII/NotI. Cut out top band and saved for purification tomorrow. Transformation of pacBB Eut...)
• 00:26, 24 October 2010 (diff | hist) N Minnesota-experimental/15 July 2010 ‎ (New page: '''Annie''' Minipreps of pacBBGGFP. Two samples sent for sequencing at University of Minnesota BioMedical Genomics Center. '''Ian''' PCR purification of LKMNS insert.) (top)
• 00:22, 24 October 2010 (diff | hist) N Minnesota-experimental/14 July 2010 ‎ (New page: '''Ian''' Minipreps set up of overnight cultures. Ligation reaction set up of pacBB-EutC-DsRed. Digestion reaction set up of pucBB-Eut LKMNS.) (top)
• 00:21, 24 October 2010 (diff | hist) Minnesota-experimental/13 July 2010 ‎ (top)
• 00:20, 24 October 2010 (diff | hist) N Minnesota-experimental/13 July 2010 ‎ (New page: '''Annie''' Transformations of pacBB and GGFP ligation reaction on CM plates. Digestion of pucBB GGFP with BglII/NotI overnight at thirty seven degree incubator. '''Matt''' Minipreps of T...)
• 00:16, 24 October 2010 (diff | hist) N Minnesota-experimental/12 July 2010 ‎ (New page: '''Annie''' Set up pacBB GGFP ligation reaction (BglII/NotI). '''Matt''' Overnight cultures of pacBB-EutG-dsRed overnight at thirty seven degrees Celsius incubator. '''Tony''' Ligation of ...) (top)
• 00:13, 24 October 2010 (diff | hist) N Minnesota-experimental/9 July 2010 ‎ (New page: '''Annie''' Ran gel pucBB GGFP digest and GGFP. '''Tony''' Purified digested Eut C DsRed BglII/NotI fragment. Eluted in thirty microliter buffer EB. '''Ian''' Made nine CM plates. Transf...) (top)
• 00:11, 24 October 2010 (diff | hist) N Minnesota-experimental/8 July 2010 ‎ (New page: '''Annie''' Minipreps of pucBB GGFP. Nanodrop concentrations of pucBB GGFP. Digestion of pucBB GGFP with BglII/NotI. '''Tony and Ian''' Nanodrop concentration of pacBB EutG DsRed. Ligat...) (top)
• 00:08, 24 October 2010 (diff | hist) Minnesota-experimental/7 July 2010 ‎ (top)
• 00:04, 24 October 2010 (diff | hist) N Minnesota-experimental/7 July 2010 ‎ (New page: '''Annie''' PCR reactions set up for EutC and EutG. Overnight cultures set up for pucBB GGFP in thirty seven degree incubator.)
• 00:01, 24 October 2010 (diff | hist) N Minnesota-experimental/6 July 2010 ‎ (New page: '''Annie''' Set up transformations for all three ligations (pac EutC DsRed, pacBB EutG DsRed, pacBB EutC GFP).) (top)
• 00:00, 24 October 2010 (diff | hist) N Minnesota-experimental/1 July 2010 ‎ (New page: '''Annie''' PCR purification of Eut G DsREd, Eut C DsRed, and Eut G GFP. '''Tony''' Minipreps of pacBB plasmid. Eluted in forty microliters DH2O (four tubes). '''Ian''' Nanodrop concentra...) (top)
• 23:57, 23 October 2010 (diff | hist) N Minnesota-experimental/30 June 2010 ‎ (New page: '''Annie''' PCR purification of Eut C DsRed and Eut G DsRed from previous day digestion. PCR reaction of of EutC dsRed. Digestion reaction of Eut G GFP with BglII/NotI. '''Ian''' Ran gel...) (top)
• 23:45, 23 October 2010 (diff | hist) N Minnesota-experimental/29 June 2010 ‎ (New page: '''Annie''' Minipreps of pac and GFP. Overnight digest of DsREd Eut C and Eut G with BglII/NotI. '''Matt''' Digestion of pacBB-GFP with BglII/NotI (fifty microliter reaction). Placed in ...) (top)
• 23:42, 23 October 2010 (diff | hist) Minnesota-experimental/28 June 2010 ‎ (top)
• 23:41, 23 October 2010 (diff | hist) Minnesota-experimental/28 June 2010 ‎
• 23:40, 23 October 2010 (diff | hist) N Minnesota-experimental/28 June 2010 ‎ (New page: '''Annie''' Ran PCR reaction of pac-DsRed. Overnight culture of ligation.)
• 23:39, 23 October 2010 (diff | hist) Minnesota-experimental/24 June 2010 ‎ (top)
• 23:35, 23 October 2010 (diff | hist) N Minnesota-experimental/24 June 2010 ‎ (New page: '''Annie''' Redo of failed PCR reaction (Eut C-DsREd Express 2). Ligation reaction set up to confirm.)
• 23:33, 23 October 2010 (diff | hist) Minnesota-experimental/23 June 2010 ‎ (top)
• 23:28, 23 October 2010 (diff | hist) N Minnesota-experimental/23 June 2010 ‎ (New page: '''Annie''' Vector digestion with BglII/NotI. Placed in thirty seven degree incubator overnight. PCR product of Eut-C DsRed product ran on gel. Gel failed. '''Ian''' PacBB-GFP digest wi...)
• 23:22, 23 October 2010 (diff | hist) Minnesota-experimental/22 June 2010 ‎ (top)
• 23:20, 23 October 2010 (diff | hist) N Minnesota-experimental/22 June 2010 ‎ (New page: '''Tony''' PCR of Eut C-DsREd Express2. Minipreps of PCR and eluted with fifty microliters EB buffer.)
• 23:18, 23 October 2010 (diff | hist) N Minnesota-experimental/21 June 2010 ‎ (New page: '''Tony, Annie, and Matt''' Made LB plates with CM and AMP. Cultivated pacBB and pucBB-Eut G-GFP colonies in liquid media. PCR reaction of Psb3K5 DsRed plasmid template.) (top)
• 23:14, 23 October 2010 (diff | hist) N Minnesota-experimental/17 June 2010 ‎ (New page: '''Annie''' Transformation of Eut GGFP into competent cells. Transformation of pac into competent cells. '''Ian and Tony''' Transformation of E. Coli with pac and pucmod GGFP.) (top)
• 23:13, 23 October 2010 (diff | hist) N Minnesota-experimental/16 June 2010 ‎ (New page: '''Matt''' Nanodrop concentrations of gel purified pac and GGFP. Ran gel of pac and GGFP. Gel did not show any bands (possible failure of ethidium bromide staining). Need to perform ano...) (top)
• 23:11, 23 October 2010 (diff | hist) N Minnesota-experimental/15 June 2010 ‎ (New page: '''Annie''' Gel purification followed by ligation reaction of pac and GGFP. '''Ian''' Made CM plates. Transformation of pac GeGFP. Plated overnight in thirty seven degree Celsius incubat...) (top)
• 23:09, 23 October 2010 (diff | hist) N Minnesota-experimental/14 June 2010 ‎ (New page: '''Tony''' Insert pLac EutG GFP into pacBB. Digestion reaction of fifteen microliters of pucBB Eut G GFP with EcoRI/PstI.) (top)
• 23:05, 23 October 2010 (diff | hist) N Minnesota-experimental/8 June 2010 ‎ (New page: '''Ian''' Sent pucmod BB GeGFP for sequencing to University of Minnesota BioMedical Genomics Center. PCR reaction of GeGFP. '''Annie''' Ran gel of PCR product for sequencing.) (top)
• 23:03, 23 October 2010 (diff | hist) Minnesota-experimental/7 June 2010 ‎ (top)
• 22:32, 23 October 2010 (diff | hist) N Minnesota-experimental/7 June 2010 ‎ (New page: '''Annie''' Digestion reaction of GGFP with EcoRI/PstI.)
• 22:32, 23 October 2010 (diff | hist) N Minnesota-experimental/4 June 2010 ‎ (New page: '''Tony and Ian''' Set up overnight cultures of GGFP.)
• 22:29, 23 October 2010 (diff | hist) N Minnesota-experimental/3 June 2010 ‎ (New page: '''Annie''' Transformations of pucmod BB and pac BB. Plated overnight on ampicillin plates. '''Tony and Matt''' Concentrations of GGFP BglII/Not I and pucBB BglII/Not I. Twenty five micr...)
• 22:21, 23 October 2010 (diff | hist) N Minnesota-experimental/2 June 2010 ‎ (New page: '''Annie''' Ran out GGFP PCR product on gel. Digestion reaction of gel product with BglII/NotI. Minipreps of pucBB completed. Digestion reaction of pucBB reactions with BglII/Not I. Ra...)
• 22:17, 23 October 2010 (diff | hist) Minnesota-experimental/1 June 2010 ‎
• 22:15, 23 October 2010 (diff | hist) Minnesota-experimental/1 June 2010 ‎
• 22:14, 23 October 2010 (diff | hist) Minnesota-experimental/1 June 2010 ‎
• 22:13, 23 October 2010 (diff | hist) Minnesota-experimental/28 May 2010 ‎ (top)
• 22:11, 23 October 2010 (diff | hist) N Minnesota-experimental/28 May 2010 ‎ (New page: '''Annie''' Minipreps of PAC and TCM. Digestion to be done on Monday.)
• 22:10, 23 October 2010 (diff | hist) Minnesota-experimental/27 May 2010 ‎ (top)
• 22:08, 23 October 2010 (diff | hist) N Minnesota-experimental/27 May 2010 ‎ (New page: '''Annie''' Ran out gel of previous digest. '''Annie, Tony, and Matt''' Ligation reaction of cut digestion product. '''Matt''' Overnight cultures of previous day digestion reaction. '''Ann...)
• 21:57, 23 October 2010 (diff | hist) N Minnesota-experimental/26 May 2010 ‎ (New page: '''Tony''' PCR reactions of pucBB eGFP. '''Ian''' Digestion reaction of pucBB eGFP with BglII/NotI.) (top)
• 21:55, 23 October 2010 (diff | hist) N Minnesota-experimental/7 May 2010 ‎ (New page: '''Annie''' Preparation of competent cells. '''Ian''' Digestions of Eut C and G with BglII/NotI. Gel purification of cGFP and gGFP.)
• 21:52, 23 October 2010 (diff | hist) N Minnesota-experimental/6 May 2010 ‎ (New page: '''Annie''' PCR reaction of pucBB eGFP-Eut G.)
• 21:50, 23 October 2010 (diff | hist) N Minnesota-experimental/5 May 2010 ‎ (New page: '''Tony and Ian''' Concentrations takeon of EutC-EGFP and EutG-GFP. Eut SMNLK, Eut SMNLK, EutC eGFP, and EutG eGFP submitted to University of Minnesota BioMedical Genomics Center for DNA ...)
• 21:42, 23 October 2010 (diff | hist) N Minnesota-experimental/4 May 2010 ‎ (New page: '''Ian''' Minipreps of CGFP and GGFP. Stored in negative twenty degree freezer.)

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