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From 2010.igem.org

Annie PCR reactions set up for EutC and EutG. Overnight cultures set up for pucBB GGFP in thirty seven degree incubator. Tony Gel of PCR run and purified and eluted EutG-DsREd fragment. Second attempt of Eut C PCR. Digestion reaction of EutG DsREd and pacBB overnight with BglII/NotI.