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From 2010.igem.org

Annie Minipreps of pucBB GGFP. Nanodrop concentrations of pucBB GGFP. Digestion of pucBB GGFP with BglII/NotI. Tony and Ian Nanodrop concentration of pacBB EutG DsRed. Ligation reaction of pacBB with insert. Eut C digestion reaction with BglII/NotI.