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From 2010.igem.org

Annie Transformations of pacBB and GGFP ligation reaction on CM plates. Digestion of pucBB GGFP with BglII/NotI overnight at thirty seven degree incubator. Matt Minipreps of Tony's pacBB-EutG-DsRed ligation. Prepared and sent for sequencing to University of Minnesota BioMedical Genomics Center. Digestion reaction set up of pacBB with BglII/NotI Ian Transformations of pucBB Eut LKMNS plasmids. Overnight cultures set up.