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Team:Newcastle/13 August 2010
From 2010.igem.org
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Contents |
rocF BioBrick miniprep
rocF BioBrick nanodrop
rocF BioBrick gel electrophoresis
Expect band to be 3195bp.
Subtilin Immunity BioBrick
Aims
We plan to select three spaIFEG gene (part 3) PCR tubes, using Tms 51°C, 56°C and 61°C (we had confirmed it successful from yesterday's gel electrophoresis). Gel extraction will then be performed, along with Plasmid Vector (part 1), Promoter & RBS (part 2) and Double terminator (part 4). Finally, NanoDrop will be performed.
Materials and protocol
Please refer to the gel electrophoresis, gel extraction and NanoDrop protocols.
Results
Discussion
We chose the three spaIFEG gene (part 3) PCR tubes, using Tms 51°C, 56°C and 61°C because they were the mid-ranged Tms. Gel extraction was then performed, by firstly cutting the gels under U.V light, and then apply the protocol for completion.
Conclusion
The results from the NanoDrop reading suggests that our results were positive, which indicates that we have successfully extract all the parts required for Subtilin Immunity BioBrick. Gibson cloning method will be used next week.
Go back to our main Lab book page
   
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