"

From 2010.igem.org

---

July	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31
August	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31
September	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30
October	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31

We picked two colonies for each the following parts

2a, 3a, 3b, 6a, 6b, 9a, 9b, 11a, 11b, 14a, 14b, 15a, 15b, 20a, 
20b, 21a, 21b, 27a, 27b, 28a, 28b, 29a, 29b, 30a, 30b, 31a, 33a, 33b

The gel electrophoresis of the colony PCRs didn't run as expected. We have to repeat it on Monday. We decided anyhow the purify the plasmids tomorrow.

For the PCR we used the [pcr protocol] protocol. For the gel electrophoresis we use 1% and 2% agarose gels with [http://openwetware.org/wiki/TBE TBE buffer].