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BIOTEC Dresden/Notepad/27 August 2010
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Gel purification for the purified digests was done. Only bands corresponding to the backbone size were seen and the bands denoting the insert lengths were not seen. This may have been due to running the gel for too long or most of the DNA was lost during the purification step after the digest.
Fusion Protein
All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. The plasmids pETMM43 and pETMMZZ were double digested and purified from agarose gel.
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