Team:BIOTEC Dresden/Protocols:Cocultivation assay

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Revision as of 17:03, 26 October 2010 by Lucas.schirmer (Talk | contribs)

Set-up

Tecan plate reader

  • Start the machine 20 minutes in advance and heat the chamber to 37C. Put in the plate and start the measurement with the following setting:
  • Measure OD at 612 nm
  • Measure fluorescence with an excitation wavelength of 485nm and an emission wavelength of 535nm for both GFP and YFP
  • Shake
  • Repeat measurements every 5min for 3 hours

Data processing

  • Normalize the data by dividing all fluorescence data by the corresponding optical density
  • Subtract the obtained value of the reference column from the calculated relative fluorescence of the wells containing HHL
  • Plot the fluorescence data over the time
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