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Team:Peking/Notebook/ALiu
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Ao Liu's Notes
I Conducted mutagenesis library for Mer OP and research on the feature of promoter functional sites through screening the library (characterizging promoters with different dyad MerR binding site). Then promoters with different Hg binding affinity was integrated with the results gained from operation analysis to acquire the wider range of bioreproter Hg-sensitivity.
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[TOP]
6.24
Transform plasmids to Trans5a. pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3, pSB1C3, pSB1K3, pSB1T3, pSB3C5, pSB3T5, pSB4C5, pSB4K5, pMAL-C4X, pET-39b.
6.26
Prepare plasmid DNA. pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3, pSB1C3, pSB1K3, pSB1T3, pSB3C5, pSB3T5, pSB4C5, pSB4K5, pMAL-C4X, pET-39b
Digest the plasmid DNA with EcoRI &PstI.
Retrieve the digested product
6.27
Transform plasmids to Trans5a.1-12O, pSD-MBD, pASK-MBD, NRI.
6.28
Picking colonies and shaking at 37℃ overnight. 1-12O
Prepare plasmid DNA. pSD-MBD, pASK-MBD, NRI.
6.29
PCR Strain NRI with two pairs of primers
6.30
PCR Strain NRI with two pairs of primers
Retrieve the PCR product
Prepare plasmid DNA. 1-12O
