2010/09/28

1：DNA extraction

＜Member＞
mizuki,Takahiro

＜Sample＞
PCR products

1(9/16)
2(9/16)
3(9/17)
4(9/16)
5(9/16)
7(9/16)

＜Protocol＞
See Protocol 4

2：DNA Ligation

＜Member＞
mio

＜Sample＞

7-8-10,vector
2,vector

(after digestion)

＜Protocol＞
See Protocol 3

3:PCR

<Member>
mizuki,Takahiro

<Sample>(temperature in annealing:℃)

1 (72)
2 (70)
4 (70)
5 (70)
9 (63,68)
10(58)
vector(72)

<Protocol>
See Protocol 1

4:Transformation

<Member>
nito

7-8-10+vector
2+vector

<Protocol>
See Protocol 7

5:Electrophoresis

<Member>
nito,Takahiro

<Sample>
PCR products

1 (9/28)
2 (9/28)
4 (9/28)
5 (9/28)
8 (9/27)
9 (9/28)
10(9/28)
vector

<Protocol>
See Protocol 8

6：DNA extraction

＜Member＞
Takahiro

＜Sample＞
PCR products

＜Protocol＞
See Protocol 4

7：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR productions

1 (8/31)
2 (9/11)
4L(8/26)
5 (8/31)

＜Protocol＞
See Prptpcpl 9

8:PCR

<Member>
Mariko