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BIOTEC Dresden/Notepad/20 October 2010
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Parts Assembly
The digested parts were ligated into the plasmid backbone containing chloramphenicol.
52b, 52c, 53a, 53c, 54a, 54b, 54c, 55a, 55b, 55c
The ligation mix was heat inactivated at 65 degrees for 15 min. The mix was then transformed by chemical transformation method and plated for overnight growth of colonies.
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