Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/28
From 2010.igem.org
Contents |
2010/09/28
1:DNA extraction
<Member>
mizuki,Takahiro
<Sample>
PCR products
<Protocol>
See Protocol 4
2:DNA Ligation
<Member>
mio
<Sample>
(after digestion)
<Protocol>
See Protocol 3
3:PCR
<Member>
mizuki,Takahiro
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1
4:Transformation
<Member>
nito
<Sample>
<Protocol>
See Protocol 7
5:Electrophoresis
<Member>
nito,Takahiro
<Sample>
PCR products
<Protocol>
See Protocol 8
6:DNA extraction
<Member>
Takahiro
<Sample>
PCR products
<Protocol>
See Protocol 4
7:DNA Digestion
<Member>
Mariko
<Sample, Materials>
・PCR productions
- 1 (8/31)
- 2 (9/11)
- 4L(8/26)
- 5 (8/31)
・Digest enzyme
- Avr2
- Nhe1
- spe1
<Protocol>
See Prptpcpl 9
8:PCR
<Member>
Mariko
<Sample>(temperature in annealing:℃)
- 1 (72)
- 2 (70)
- 3 (69)
- 4 (70)
<Protocol>
See Protocol 1