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BIOTEC Dresden/Notepad/11 October 2010
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Parts Assembly
Thirty colonies were picked for the following part and colony PCR was done.
2e + 6e + 14f
The PCR samples were run on an agarose gel and the positive clones were identified corresponding to the bands obtained as per the desired insert length. The cultures corresponding to the positive clones were left for overnight shaking.
Fusion Protein
Colony PCR for the luxI + zz fusion protein and one colony was picked for the overnight culture
Restriction digest of the plasmids: pSB1T3 and pSB1C3
Transformation of the plasmid pETMM43
July |
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August |
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September |
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October |
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