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BIOTEC Dresden/Notepad/19 October 2010
From 2010.igem.org
Parts Assembly
All the parts need to be ligated into the plasmid backbone containing chloramphenicol as per iGeM requirement. The following parts were restriction digested with EcoRI and PstI for 1 hour 15 min at 37 degrees.
52b, 52c, 53a, 53b, 53c, 54a, 54b, 54c, 55a, 55b, 55c, backbone, 6e-4a
The digested parts were purified by gel purification and nanodropped.
Fusion Protein
50 colonies were picked from the ligation plates of the fusion protein for colony PCR. Colonies were picked for the overnight cultures.
AHL Sensor
Two plates were measured: 1751 in Ampicillin and 1751 in Chloramphenicol
Miniprep of overnight cultures of 17+51
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