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BIOTEC Dresden/Notepad/30 August 2010
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Fusion Protein
The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour. The whole ligation reaction was used for electroporation.
AHL Sensor
AHL plate reader assay was done with part 24f. A pipetting scheme was used where different concentrations of the stock solution was used (from 0.015µM to 150µM) in LB.
Measurement of part 17e for 3 hours using AHL concentrations of (0.01-2000nM)
July |
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