Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/07

From 2010.igem.org

(Difference between revisions)
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{{:Team:Tokyo_Metropolitan/Header}}
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=2010/10/07=
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===1:PCR===
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<Member><br />
 +
nito
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<Sample>(temperature in annealing:℃)<br />
 +
*1
 +
*2
 +
*3
 +
*4
 +
:5
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*6
 +
*7
 +
*8
 +
*9
 +
*10
 +
*12
 +
*45
 +
*23
 +
*27
 +
*67
 +
*210
 +
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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 +
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===2：DNA extraction===
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＜Member＞<br />
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Takahiro
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 +
＜Sample＞<br />
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PCR products (10/6)
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 +
＜Protocol＞<br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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 +
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==3:Plasmid extraction from E-coli===
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<Member><br />
 +
nito,Taka
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 +
<Sample><br />
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*
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*
 +
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]
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 +
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===4:Electrophoresis===
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<Member><br />
 +
nito
 +
 +
<Sample><br />
 +
PCR products (10/7)
 +
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8
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<Result><br />
[[Image:Matsuura_2010-10-07_21hr_54min.JPG]]<br />
[[Image:Matsuura_2010-10-07_21hr_54min.JPG]]<br />
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 +
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===5：DNA extraction===
 +
＜Member＞<br />
 +
mizuki,mio
 +
 +
＜Sample＞<br />
 +
PCR products
 +
*1
 +
*4
 +
*5
 +
*9
 +
*10
 +
*12
 +
*210
 +
 +
＜Protocol＞<br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
 +
 +
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===6：DNA extraction from gels===
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＜Member＞<br />
 +
nito
 +
 +
＜Sample＞<br />
 +
Digestion products
 +
*9
 +
*12
 +
*210
 +
(After electrophoresis)
 +
 +
＜Protocol＞<br />

Revision as of 05:26, 8 October 2010


Contents

2010/10/07

1:PCR

<Member>
nito

<Sample>(temperature in annealing:℃)

  • 1
  • 2
  • 3
  • 4
5
  • 6
  • 7
  • 8
  • 9
  • 10
  • 12
  • 45
  • 23
  • 27
  • 67
  • 210

<Protocol>
See Protocol 1


2：DNA extraction

＜Member＞
Takahiro

＜Sample＞
PCR products (10/6)

＜Protocol＞
See Protocol 4


3:Plasmid extraction from E-coli=

<Member>
nito,Taka

<Sample>

<Protocol>
See Protocol 6


4:Electrophoresis

<Member>
nito

<Sample>
PCR products (10/7)

<Protocol>
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8

<Result>
Matsuura 2010-10-07 21hr 54min.JPG


5：DNA extraction

＜Member＞
mizuki,mio

＜Sample＞
PCR products

  • 1
  • 4
  • 5
  • 9
  • 10
  • 12
  • 210

＜Protocol＞
See Protocol 4


6：DNA extraction from gels

＜Member＞
nito

＜Sample＞
Digestion products

  • 9
  • 12
  • 210

(After electrophoresis)

＜Protocol＞

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/07"