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Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/28
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(New page: ===1：DNA extraction=== ＜Member＞<br /> mizuki,Takahiro ＜Sample＞<br /> PCR products *1(9/16) *2(9/16) *3(9/17) *4(9/16) *5(9/16) *7(9/16) ＜Protocol＞<br /> See [http://2010.ig...) |
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===1：DNA extraction=== | ===1：DNA extraction=== | ||
＜Member＞<br /> | ＜Member＞<br /> | ||
Revision as of 13:31, 29 September 2010
Contents |
2010/09/28
1：DNA extraction
＜Member＞
mizuki,Takahiro
＜Sample＞
PCR products
- 1(9/16)
- 2(9/16)
- 3(9/17)
- 4(9/16)
- 5(9/16)
- 7(9/16)
＜Protocol＞
See Protocol 4
2：DNA Ligation
＜Member＞
mio
＜Sample＞
- 7-8-10,vector
- 2,vector
(after digestion)
＜Protocol＞
See Protocol 3
3:PCR
<Member>
mizuki,Takahiro
<Sample>(temperature in annealing:℃)
- 1 (72)
- 2 (70)
- 4 (70)
- 5 (70)
- 9 (63,68)
- 10(58)
- vector(72)
<Protocol>
See Protocol 1
4:Transformation
<Member>
nito
<Sample>
- 7-8-10+vector
- 2+vector
<Protocol>
See Protocol 7
5:Electrophoresis
<Member>
nito,Takahiro
<Sample>
PCR products
- 1 (9/28)
- 2 (9/28)
- 4 (9/28)
- 5 (9/28)
- 8 (9/27)
- 9 (9/28)
- 10(9/28)
- vector
<Protocol>
See Protocol 8
<Result>
[[Image:]]
6：DNA extraction
＜Member＞
Takahiro
＜Sample＞
PCR products
＜Protocol＞
See Protocol 4
7：DNA Digestion
＜Member＞
Mariko
＜Sample, Materials＞
・PCR productions
- 1 (8/31)
- 2 (9/11)
- 4L(8/26)
- 5 (8/31)
・Digest enzyme
- Avr2
- Nhe1
- spe1
＜Protocol＞
See Prptpcpl 9
8:PCR
<Member>
Mariko
<Sample>(temperature in annealing:℃)
- 1 (72)
- 2 (70)
- 3 (69)
- 4 (70)
<Protocol>
See Protocol 1
