Team:Caltech/Week 13

(Difference between revisions)

Revision as of 20:44, 20 September 2010

iGEM 2010

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support

Colony PCR cultures of L95 (HSP-LacI-EYFP) cells imaged under microscope
- Cells are vastly overgrown and unhealthy (cultures visualized after one weekend)
Fresh cultures of L95 cells grown in preparation for a proper microscope imaging session

Digested successful sigma32 (HtpR) BioBrick in preparation for ligation into pSB1C3 chloramphenicol backbone
- Treat the digestion like a backbone digestion
Ligated the digest into pSB1C3
Cleaned up the lab in preparation for mandatory leave of absence

Photographed the results of transglutaminase long-term testing (6 week span)
- Results are stunning: TGase + cells, TGase + cell lysate forms a dark grey-colored brick about 7mm diameter (culture tube was approximately 13-14mm inside diameter!)
- Several TGase + acid and TGase + base show very dark, vivid colors unattributable to the acid/base
Mandatory leave of absence begins

Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found

@@ Line 18: / Line 18: @@
 ==Wednesday 9/8==
+*Photographed the results of transglutaminase long-term testing (6 week span)
+**Results are stunning: TGase + cells, TGase + cell lysate forms a dark grey-colored brick about 7mm diameter (culture tube was approximately 13-14mm inside diameter!)
+**Several TGase + acid and TGase + base show very dark, vivid colors unattributable to the acid/base
 *Mandatory leave of absence begins