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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/30
From 2010.igem.org
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| - | ===Experiment:separation of ''A. | + | ===Experiment:separation of ''A.xylinum''=== |
'''member'''<br/> | '''member'''<br/> | ||
naoto | naoto | ||
'''material'''<br/> | '''material'''<br/> | ||
| - | *''A. | + | *''A.xylinum'' in open wet ware media(8/25 made) |
'''procedure'''<br/> | '''procedure'''<br/> | ||
Revision as of 15:21, 16 September 2010
Contents |
2010/8/30 Monday (Naoto)
Experiment:electrophoresis
member
NEX,bambi75 and watachin
material
- PCR production (bcsC from E.coli)
- 1×TAE buffer
- agarose gel
procedure
follow to protocol4
result
bands of bcsC were appeared
Experiment:separation of A.xylinum
member
naoto
material
- A.xylinum in open wet ware media(8/25 made)
procedure
- take 0.5ml of A.xylinus in open wet ware media to tubes
- centrifuge 15000rpm/5min
Experiment:direct PCR
member
naoto
material
- A.xylinum separated above experiment
procedure
follow to protocol3
Experiment:purification of agarose gel
member
NEX,bambi75 and watachin
material
- bcsC from E.coli in agarose gel
- QIAGEN
procedure
follow to protocol5
Experiment:electrophoresis
member
naoto
material
- PCR production (bcsA from A.xylinum)
- 1×TAE buffer
- agarose gel
procedure
follow to protocol4
result
a band of bcsA were appeared
(below bands appreared to be primer)
Experiment:purification of agarose gel
member
naoto
material
- bcsA from A.xylinum in agarose gel
- QIAGEN
procedure
follow to protocol5
