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Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/30
From 2010.igem.org
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'''material'''<br/> | '''material'''<br/> | ||
| - | *''A. | + | *''A.xylinum'' separated above experiment |
'''procedure'''<br/> | '''procedure'''<br/> | ||
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'''material'''<br/> | '''material'''<br/> | ||
| - | *PCR production (bcsA from A. | + | *PCR production (bcsA from A.xylinum) |
*1×TAE buffer | *1×TAE buffer | ||
*agarose gel | *agarose gel | ||
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'''material'''<br/> | '''material'''<br/> | ||
| - | *bcsA from ''A. | + | *bcsA from ''A.xylinum'' in agarose gel |
*QIAGEN | *QIAGEN | ||
'''procedure'''<br/> | '''procedure'''<br/> | ||
follow to protocol5 | follow to protocol5 | ||
Revision as of 15:19, 16 September 2010
Contents |
2010/8/30 Monday (Naoto)
Experiment:electrophoresis
member
NEX,bambi75 and watachin
material
- PCR production (bcsC from E.coli)
- 1×TAE buffer
- agarose gel
procedure
follow to protocol4
result
bands of bcsC were appeared
Experiment:separation of A.xylinus
member
naoto
material
- A.xylinus in open wet ware media(8/25 made)
procedure
- take 0.5ml of A.xylinus in open wet ware media to tubes
- centrifuge 15000rpm/5min
Experiment:direct PCR
member
naoto
material
- A.xylinum separated above experiment
procedure
follow to protocol3
Experiment:purification of agarose gel
member
NEX,bambi75 and watachin
material
- bcsC from E.coli in agarose gel
- QIAGEN
procedure
follow to protocol5
Experiment:electrophoresis
member
naoto
material
- PCR production (bcsA from A.xylinum)
- 1×TAE buffer
- agarose gel
procedure
follow to protocol4
result
a band of bcsA were appeared
(below bands appreared to be primer)
Experiment:purification of agarose gel
member
naoto
material
- bcsA from A.xylinum in agarose gel
- QIAGEN
procedure
follow to protocol5
