Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/30

From 2010.igem.org

(Difference between revisions)
(2010/8/30 Monday (Naoto))
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'''material'''<br/>
'''material'''<br/>
-
*''A.xylinus'' separated above experiment
+
*''A.xylinum'' separated above experiment
'''procedure'''<br/>
'''procedure'''<br/>
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'''material'''<br/>
'''material'''<br/>
-
*PCR production (bcsA from A.xylinus)
+
*PCR production (bcsA from A.xylinum)
*1×TAE buffer
*1×TAE buffer
*agarose gel
*agarose gel
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'''material'''<br/>
'''material'''<br/>
-
*bcsA from ''A.xylinus'' in agarose gel
+
*bcsA from ''A.xylinum'' in agarose gel
*QIAGEN
*QIAGEN
'''procedure'''<br/>
'''procedure'''<br/>
follow to protocol5
follow to protocol5

Revision as of 15:19, 16 September 2010


Contents

2010/8/30 Monday (Naoto)

Experiment:electrophoresis

member
NEX,bambi75 and watachin

material

  • PCR production (bcsC from E.coli)
  • 1×TAE buffer
  • agarose gel

procedure
follow to protocol4

result
bands of bcsC were appeared
201008301.jpg

Experiment:separation of A.xylinus

member
naoto

material

  • A.xylinus in open wet ware media(8/25 made)

procedure

  1. take 0.5ml of A.xylinus in open wet ware media to tubes
  2. centrifuge 15000rpm/5min

Experiment:direct PCR

member
naoto

material

  • A.xylinum separated above experiment

procedure
follow to protocol3

Experiment:purification of agarose gel

member
NEX,bambi75 and watachin

material

  • bcsC from E.coli in agarose gel
  • QIAGEN

procedure
follow to protocol5

Experiment:electrophoresis

member
naoto

material

  • PCR production (bcsA from A.xylinum)
  • 1×TAE buffer
  • agarose gel

procedure
follow to protocol4

result
a band of bcsA were appeared (below bands appreared to be primer)
201008302.jpg

Experiment:purification of agarose gel

member
naoto

material

  • bcsA from A.xylinum in agarose gel
  • QIAGEN

procedure
follow to protocol5