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BIOTEC Dresden/Notepad/30 August 2010
From 2010.igem.org
(Difference between revisions)
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'''Fusion Protein''' | '''Fusion Protein''' | ||
| - | The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. | + | The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour. |
| + | The whole ligation reaction was used for electroporation. | ||
Revision as of 07:53, 13 September 2010
Fusion Protein
The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour. The whole ligation reaction was used for electroporation.
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